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pet21(+) expression vector  (Merck & Co)
90
Merck & Co pet21(+) expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21(+) Expression Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet21/bio_rxiv__2024__12__02__626473-51-5-6
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pet21(+) expression vector - by Bioz Stars, 2026-11
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pet21 expression vector  (Twist Bioscience)
95
Twist Bioscience pet21 expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Expression Vector, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/Affinity/pmc11342335-64-21-24
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pet21 expression vector - by Bioz Stars, 2026-11
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pet21-b(+) expression vectors  (GenScript corporation)
90
GenScript corporation pet21-b(+) expression vectors
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 B(+) Expression Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet21b/pmc10940687-220-12-16
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pet21-b(+) expression vectors - by Bioz Stars, 2026-11
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pet21-pelb e. coli periplasmic expression vector  (Agilent technologies)
90
Agilent technologies pet21-pelb e. coli periplasmic expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Pelb E. Coli Periplasmic Expression Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/bio_rxiv__2023__10__22__563475-183-11-26
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pet21-pelb e. coli periplasmic expression vector - by Bioz Stars, 2026-11
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pet21 expression vector  (Millipore)
90
Millipore pet21 expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet28a/pm36964198-339-7-9
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pet21 expression vector - by Bioz Stars, 2026-11
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pet21-b expression vector  (Millipore)
90
Millipore pet21-b expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 B Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet28a/pmc03055897-51-15-18
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pet21-b expression vector - by Bioz Stars, 2026-11
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expression vector pet21 d  (Millipore)
90
Millipore expression vector pet21 d
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Expression Vector Pet21 D, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet28a/pm10725558-72-33-39
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expression vector pet21 d - by Bioz Stars, 2026-11
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pet21-c expression vector  (Millipore)
90
Millipore pet21-c expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 C Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet28a/pmc09321382-131-7-10
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pet21-c expression vector - by Bioz Stars, 2026-11
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protein expression vector pet21-d  (Millipore)
90
Millipore protein expression vector pet21-d
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Protein Expression Vector Pet21 D, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet28a/pmc08791256-295-13-17
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protein expression vector pet21-d - by Bioz Stars, 2026-11
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A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet: A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: Incubation

SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet: SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: SDS Page, Construct, Plasmid Preparation, Positive Control, Marker, Control

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet:

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: